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KMID : 0359320100500040331
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Korean Journal of Veterinary Research
2010 Volume.50 No. 4 p.331 ~ p.333
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Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium
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Casiano Choresca
Dennis K. Gomez
Han Jee-Eun
Shin Sang-Phil
Kim Ji-Hyung
Jun Jin-Woo
Park Se-Chang
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Abstract
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Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.
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KEYWORD
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aerolysin gene, Aeromonas hydrophila, catfish, extracellular hemolysin gene
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